Contact with the edge of the plate can introduce contaminates to the agar. Not burning the loop 2. H ow can a streak plate become contaminated? The phages that have been lysogenic have become lytic. 2. 1. The inoculum source can be a broth or solid culture, an environmental swab, a clinical specimen, or sedimented urine. 5. If it is left open on the bench it can be contaminated by bacteria in tyhe air, e.g. 8. micrococci. If it is left open on the bench it can be contaminated by bacteria in tyhe air, e.g. Tip Use a magnifying glass to help identify minute differences in bacterial colony morphology. Pelczar and Reid (8) presented a streak plate exercise. Science Biology Microbiology Epidemiology. They allow bacteria and fungi to grow on a semi-solid surface to produce discrete colonies. What is the purpose of doing a streak plate? Get solutions Get solutions Get solutions done loading Looking for the textbook? How do you streak bacteria on agar plates? This plate is called an isolation plate. Agar streak plates are an essential tool in microbiology. Streak the Plate: The lid of the agar plate has to be opened just sufficiently enough to streak the plate with the inoculation loop. Micro Lab Unit 2 (Part I) 28 terms. The contents of a glass jar that breaks in transport can also contaminate nearby foods. See to it that the plate's surface is free of droplets of condensed moisture. Remember to streak the plate for isolation as you learned in labs 2 . In the study of microorganisms, a mixed culture is one that contains more than one type of organism growing in a sterile medium, such as agar. It utilized a 4-quadrant streak pattern, and the procedure described using both a loop and a needle in the streak and all streaks were in the same direction, rather than both back and forth. If you drop the plate. Flame the loop and cool it in the agar. The streak plate method is based on dilution during the process of mechanical spreading of inoculum over the surface of solidified culture media in order to obtain well-isolated colonies of the sample at the terminal streaks. Not burning the loop Breathe on sample Leave lid off Not burn other utensils Abdullah. idontevenknow1517. Replace O-rings after 10-20 sterilization cycles to ensure a good seal. How can you tell if a streak plate is contaminated? Explain. . We can accidently contaminate the streak plate by forgetting sterilize the loop while streaking and leaving the lid off to let the pathogens outside move in. Flame the loop again and allow it to cool. control. If you completely remove the lid, it can become contaminated with bacteria from the environment. Immediately streak the inoculating loop gently over a quarter of the plate using a back-and-forth motion (see area 1 in the figure above). If lid isn't on. Breathe on sample 3. Chemical spillages can also contaminate products. 28 terms. Follow these best practices to get your streaking technique down to a science. Expressing these chromoproteins in E. coli would make it easy to distinguish colonies from one another in the challenge. 0. They're made up of cartilage, a rubbery, flexible material (the nose, for instance, is made of cartilage). 0. It will be much easier to tell on plate 2 or 3. It is much better to store media at room temperature and detect contamination before the medium is used. Sets with similar terms. Once isolated using the streak plate, the bacterium may be identified through sequencing of its 16S gene or through. when streaking plates Posted July 20, 2022 Blog Editor Avoid the edge. If the loop was not flamed adequately before. If the plate has not been inoculated, the presence of any bacterial colonies indicates contamination. How might your streak plate become contaminated with another colony type? How would you ensure your antibiotic is working correctly for the bacterial strain you are using? Do not forget the O-rings on and in sensors. Hint. Wipe excess water from outside of bottle._ (1 mark) Download. 7/ How can a streak plate become contaminated? Avoid touching the edge the plate with the loop while streaking. Consider this step 2. The loop is sterilized after the initial inoculum is put on to avoid contamination of other microbes or impurities. Even if it's clean in appearance you don't want to bring along any contamination that may not have germinated that could have been in your spores. Sprizzle. Gently tip to mix agar. If it is left open on the bench it can be contaminated by bacteria in tyhe air, e.g. The first section will have the most because it is containing the entire loop full of bacteria. Three Sector Streak (t streak): Sterilize the wire loop. Overlap the step 1 streak 3-4 times to pull out a reduced number of bacteria, and spread them out down the side of the plate. How can streak plate become contaminated? How might your streak plate become contaminated with another colony type? 0. alexandra. If the loop was not flamed adequately before. 10 Write a brief explanation of the following terms: a. Inoculate b. J@sonLee. How can a streak plate become contaminated? List at least three different ways. Label first, streak second. Make sure you streak lightly so as not to gouge the agar. How could your streak plate method be improved? Question: 2. How can a culture become contaminated? If the loop is not sterilized. Returning to the edge of area 1 that you just streaked, extend the streaks into the second quarter of the plate (area 2). How can a streak plate become contaminated? Inspect for signs of bacterial contamination. How can a streak plate become contaminated provide at least 2 ways? Method A: Spread-plating with a turntable and glass or metal rod. If the loop is not sterilized. Keep the agar dry. ANSWER 0 christina ANSWERS: 2 friendsfreak Y ou can exhale on the plate and add your bodily bacteria, by leaving the lid off you allow air particles to settle on it which may contain bacteria, you could use a loop that has not been sterilized, there are many ways! 2. stain the culture to cross . To determine what the . 2. On an inoculated plate, look for colonies that display morphology different than what you would expect from the type of bacteria used to inoculate . After streaking each quadrant, do not forget to flame the loop. Fresh produce can be contaminated if it is loaded into a truck that was not properly cleaned and sanitised after the transporting of animals or animal products. One can streak it on a suitable agar media, it depends on what kind of bacteria, whether its a Gram positive or Gram negative (one can use McConkey, Baird parker ). 14 How can a streak plate become contaminated? micro lab test. Contact with the edge the plate can introduce contaminates the agar. Only a specific portion of the host genome can be transferred. Solutions for Chapter LR.16 Problem 5CRQ: How can a streak plate become contaminated? Sample can be either colony on solid media or suspension in broth. Then take the loop and place the lid securely back on the plate. If you drop the plate. No comments. First, the mixture must be diluted until the various individual microorganisms become separated far enough apart on an agar surface that after incubation they form visible colonies isolated from the colonies of other microorganisms. When kids are done growing, the growth plates harden into solid bone. Flame the loop again and allow it to cool. How do you know if streak plate is contaminated. Touch the loop to the edge of the colony growing on the plate. FIG. ----- Minimize the amount of agar and the length of time the agar is exposed to the environment during the streak process. Sometimes a poor fit may be the cause even if the O-ring is intact. Bright Field Light Microscope. How can a streak plate become contaminated? What are two advantages of the streak plate method? Make sure the agar plates are free of droplets of condensed moisture. The portion on either side of where the virus is integrated can be transferred. Which area of a streak plate will contain the greatest amount of growth? What are the results of a streak plate in microbiology? By separating the plate into 4 sections and taking one loop from each section to try to make isolated colonies & sterilizing the loop in between each section. Kd3036820791. ANSWERS: 2. [X] How is contamination avoided when streaking plates? Save Share. Clean thoroughly and remove all traces of solid material. You want to takenat least 1-2 more transfers from the original plate that got spores anyway. What is considered a mixed culture? How can streak plate become contaminated? micrococci. micrococci. Overlap the step 2 streak 3-4 times and spread over the surface. t he contaminat colony WILL vary in shape and possibly pigmentation from the majority of the the colonies on a streak plate. Growth plates are the areas of active, new bone growth near the ends of bones. Because the purpose of a streak plate is to obtain isolated cultures from an inoculum, consider using a fourth quadrant to further increase the probability of isolated cultures. (1) Sterilizing With Moist Heat Moist heat provided by an autoclave or pressure cooker is an efficient way to sterilize most materials. 1. The mixed culture can include multiple species of viruses, bacteria and . A drawing representing the streak pattern recommended by Pelczar and Reid (8). Clearly labeling your plates to identify the microorganism only takes a few seconds and will help you avoid a big headache later. Take from the leading edge to a new plate. 1. Answer to How can a streak plate become contaminated?. N o ! H. ANSWER. A streak plate is used in the isolation of a bacterium from a sample. Label around the edge of the bottom (not the lid) of an agar plate with at least your name, the date, the type of growth medium, and the type of organism to be plated on the medium. How can streak plate become contaminated? Make sure the vessel and port O-rings are not flattened, torn, or feathered. Provide at least 2 ways. No comments 0 nimnums What is growth plate? List at least three different ways. Not burn other utensils. Try not to discard the spent media but keep it in a plate (may be in a 60mm one), if the media turns yellow or cloudy even then, you know it is contamination! 15 Is an isolated colony a pure culture? Culture c. Incubation d. Asepsis e. Aseptic technique f Slant g. Broth h . Leave lid off 4. Procedure:_ Bulk nutrient agar prepared by mixing 100ml water, 100ml medium (Peptone ) and2 gram agar _ Put the nutrient agar to autoclave to make sure that our mixed prepared in meltedformand held at 55 celcius degree in constant temperature water bath_ Remove agar from water bath. Include the dilution factor if plating serial dilutions. How can streak plate become contaminated? ANSWERS: 3. 9. This can then be packaged with the phages and shipped out as well. Avoid the edge. How to Streak Bacteria on Agar Plates 1 Hold plates up to a bright background, and pan back and forth watching the glare on the agar 2 Bacteria looks like a cloudy film unevenly dispersed over the surface 3 Your fresh plate should be almost clear with a slight tint. No comments. (2 marks) What is the purpose of flaming in the aseptic technique? How can a streak plate become contaminated? 62 terms. haleyellow. Flame the loop and cool it in the agar. 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